SRX3425039: Vibrio owensii LKH198 Illumina library
1 ILLUMINA (NextSeq 500) run: 7.7M spots, 2.3G bases, 1.1Gb downloads
Design: High quality genomic DNA (50 µl) was sheared to 450-500 bp by focused ultrasonication in single use Covarisª snap-cap microTUBEs on a Covarisª E220 system (10% duty cycle, 2.0 intensity, 200 cycles per burst, 45 s duration). After shearing the DNA size distribution was checked on a Fragment Analyzerª CE system. Illumina¨ compatible libraries were prepared using the KAPA LTP Library Preparation kit (#KR0452, KAPA Biosystems, Boston, MA). Genomic libraries were sequenced on an Illumina NextSeq using the mid output mode (150 cycles with 150 bp paired-end reads).
Submitted by: University of Georgia
Study:
Vibrio spp. genome sequencing and assemblyshow Abstracthide AbstractVibrio spp. genome sequencing and assembly of isolates obtained from Acropora palmata and the surrounding seawater in the Florida Keys, USA
Sample:
collected from mucus of apparently healthy Acropora palmata with no white pox disease signsLibrary:
Name: CH_198
Instrument: NextSeq 500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 7.7M spots, 2.3G bases, 1.1Gb